episomal vectors (Addgene inc)
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Addgene inc
episomal vectors
Episomal Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcxlehoct3/pCXLE-hOCT3%2F4+(Plasmid+%2327076)/10__1016_slash_j__scr__2026__103916-42-3-15
Average 93 stars, based on 82 article reviews
Episomal Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcxlehoct3/pCXLE-hOCT3%2F4+(Plasmid+%2327076)/10__1016_slash_j__scr__2026__103916-42-3-15
Average 93 stars, based on 82 article reviews
episomal vectors - by Bioz Stars,
2026-10
93/100 stars
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Centrifugation:Article Title: Generation of human induced pluripotent stem cell lines derived from Wolf-Hirschhorn syndrome patients with chromosomal 4p deletion. Article Snippet: Fibroblasts were dissociated into single cells with TrypLE Express (Thermo Fisher Scientific, Waltham, MA). .. After centrifugation at 200 × g for 3 min, 6 × 105 cells were resuspended in 100 μL Buffer R containing 1 μg each of episomal plasmid vectors, Plasmid Preparation:Article Title: Generation of human induced pluripotent stem cell lines derived from Wolf-Hirschhorn syndrome patients with chromosomal 4p deletion. Article Snippet: Fibroblasts were dissociated into single cells with TrypLE Express (Thermo Fisher Scientific, Waltham, MA). .. After centrifugation at 200 × g for 3 min, 6 × 105 cells were resuspended in 100 μL Buffer R containing 1 μg each of episomal plasmid vectors, Transfection:Article Title: Establishment of two iPSC lines from healthy donor with heterozygous mutation in the SLC26A4 gene. Article Snippet: The human induced pluripotent stem cell (iPSC) lines, CSUXHEi001-A and CSUXHEi002-A, were generated from peripheral blood mononuclear cells (PBMCs).. The donors were couple and each of them has a heterozygous mutation in the SLC26A4 gene.. It manifests in their children as Enlarged vestibular aqueduct (EVA). Article Title: Generation of three induced pluripotent stem cell clones from a functional single ventricle patient carrying the BRAF c.1897 T > C variant. Article Snippet: PBMCs were isolated from whole peripheral blood samples via density gradient centrifugation using Lymphoprep Density Gradient Medium (STEMCELL Technologies, 07801) and SepMateTM-50 tubes (STEMCELL Technologies, 15450). .. After enrichment for erythroid precursor cells, 1 x 106 PBMCs were transfected with 1 μg each of Article Title: Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated with the African-specific common SCN5A polymorphism p.S1103Y: Implications for the increased sudden deaths observed in black individuals during the COVID-19 pandemic. Article Snippet: In brief, Blood media is composed of Iscove’s modified Dulbecco’s medium (IMDM; Gibco, 12440053), 20% KnockOut Serum Replacement (Gibco, Thermo Fisher Scientific, Waltham, MA, 10828010), insulinlike growth factor (IGF-1; Peprotech US, Cranbury, NJ, 100-11), erythropoietin (EPO; Peprotech, 100-64), interleukin-3 (IL-3; Peprotech, 200-03), and human stem cell factor (hSCF; Peprotech, 300-07). .. DNA for transfection was prepared with Electroporation:Article Title: Establishment of two iPSC lines from healthy donor with heterozygous mutation in the SLC26A4 gene. Article Snippet: The human induced pluripotent stem cell (iPSC) lines, CSUXHEi001-A and CSUXHEi002-A, were generated from peripheral blood mononuclear cells (PBMCs).. The donors were couple and each of them has a heterozygous mutation in the SLC26A4 gene.. It manifests in their children as Enlarged vestibular aqueduct (EVA). Article Title: Generation of an induced pluripotent stem cell line from a patient with epileptic encephalopathy caused by the CYFIP2 R87C variant. Article Snippet: Induced pluripotent stem cells (iPSCs) opened the possibility to use patient cells as a model for several diseases. iPSCs can be reprogrammed from somatic cells collected in a non-invasive way, and then differentiated into any other cell type, while maintaining the donor ́s genetic background.. CYFIP2 variants were associated with the onset of an early form of epileptic encephalopathy.. Studies with patients showed that the R87C variant seems to be one of the variants that causes more severe disease, however, to date there are no studies with a human cell model that allows investigation of the neuronal phenotype of the R87C variant. Cell Culture:Article Title: Establishment of two iPSC lines from healthy donor with heterozygous mutation in the SLC26A4 gene. Article Snippet: The human induced pluripotent stem cell (iPSC) lines, CSUXHEi001-A and CSUXHEi002-A, were generated from peripheral blood mononuclear cells (PBMCs).. The donors were couple and each of them has a heterozygous mutation in the SLC26A4 gene.. It manifests in their children as Enlarged vestibular aqueduct (EVA). |